Abstract：Using Haematococcus pluvialis as raw material, The optimal growth condition of H.pluvialis was determined by optimizing the conditions of medium, light, pH and astaxanthin. The cell wall of H.pluvialis were broken by grinding combined ultrasonic method and the astaxanthin was extracted by using ethyl acetate, acetone and 70% ethanol, respectively. The content of astaxanthin was detected by UV absorption spectroscopy, infrared absorption spectroscopy and high performance liquid chromatography. The results showed that the Haematococcus pluvialis could grow and multiply rapidly under the illumination of 2000-2500 lx for 24 hours in the modified BBM medium with pH=8.0 , and the astaxanthin was extracted by ethyl acetate after grinding. The extraction rate can reach 91.41%.In this experiment, the culture conditions of Haematococcus pluvialis were optimized, and an effective method for extracting astaxanthin was determined, which laid a foundation for the expanded production and in-depth study of Haematococcus pluvialis.
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2019, Vol. 21 
